How does HPLC column selection differ for Reb D analysis?

Zorbax SB-C18 (3.5μm, 4.6×150mm) provides 1.8x better resolution than standard C18 for Reb D/Reb M separation (Rs=2.3). Mobile phase requires 22% acetonitrile in 0.1% phosphoric acid (v/v) at 1.2mL/min for optimal peak symmetry (As=0.95-1.05).

This article was published on March 20, 2026, and last updated on March 20, 2026. The article will be continuously updated.