What is the HPLC detection method for Reb M purity?

According to GB1886.355-2022, use C18 column (4.6×150mm, 5μm) with 0.8mL/min flow rate and 210nm detection wavelength. The mobile phase requires 25-45% acetonitrile gradient in 0.1% phosphoric acid for baseline separation.

This article was published on March 20, 2026, and last updated on March 20, 2026. The article will be continuously updated.