What temperature conditions are required for Reb M sample preparation?

GB1886.355-2022 specifies 80°C extraction for 30 minutes using methanol/water (7:3 v/v) solution for optimal recovery. HPLC analysis requires column temperature at 40°C with 0.8mL/min flow rate for accurate quantification.

This article was published on March 20, 2026, and last updated on March 20, 2026. The article will be continuously updated.