What’s the standard HPLC method for STV quantification?
According to JECFA and ISO 21253-1:2019, analysis requires C18 column (4.6×250mm, 5μm) with acetonitrile/water (32:68) mobile phase at 1.0mL/min, 210nm detection. Retention times are: Stevioside (6.5min), Reb A (8.2min), Reb C (10.1min).This article was published on March 20, 2026, and last updated on March 20, 2026. The article will be continuously updated.