JECFA/WHO established an ADI of 4mg steviol equivalents/kg bw/day (equivalent to ~12mg STV/kg). This translates to approximately 720mg STV for a 60kg adult. Notably, STV has 90%+ renal excretion within 24 hours (clinical studies) and shows no mutagenicity (AMES test negative) at 5000μg/plate [1][4]. Our products carry usage guidelines compliant with all major markets including FDA GRAS and EU.

According to JECFA and ISO 21253-1:2019, analysis requires C18 column (4.6×250mm, 5μm) with acetonitrile/water (32:68) mobile phase at 1.0mL/min, 210nm detection. Retention times are: Stevioside (6.5min), Reb A (8.2min), Reb C (10.1min).

STV sweetness ranges from 200-350× sucrose depending on glycoside composition. Reb A is 200-250×, Stevioside 250-300×, and purified Reb D/M can reach 350-450×. Blends are typically formulated to match sucrose's temporal profile.

STV remains stable up to 200°C with ≤5% degradation after 1 hour. In acidic conditions (pH 3), solutions maintain >95% integrity after 6 months at 25°C. Degradation follows first-order kinetics with Ea=62.4 kJ/mol between 100-200°C.

Modern production uses: 1) Macroporous resin adsorption (AB-8/XAD-16HP), 2) Nanofiltration (200-300Da MWCO), 3) Continuous chromatography (DAC system), achieving ≥97% purity with <100ppm heavy metals and <500 TPC.

JECFA & FCC standards require: ≤1.0% moisture, ≤0.1% sulfated ash, ≤2.0% residue on ignition, lead ≤1mg/kg, arsenic ≤3mg/kg, microbial counts <1000CFU/g, and absence of solvent residues (methanol <50ppm).

Sinopure has an annual production capacity of 200 tons for high-purity steviol glycosides including STV, utilizing advanced five-stage membrane separation and continuous chromatographic purification systems . The company controls over 10,000 acres of stevia plantations producing 5,000 tons of premium dried leaves annually.

In China, STV is widely used as semi-finished product for further processing in preserved fruits (particularly in candied plums and melon seeds) due to its high stability and clean taste profile . It's also applied in bakery, dairy products, and premium beverages.

Sinopure maintains FDA GRAS, Organic, ISO9001, ISO22000, FSSC22000, HALAL, and KOSHER certifications for its steviol glycoside products including STV . The company implements integrated quality systems meeting international standards for food additives .

Our specifies HPLC analysis using C18 column (250mm length, 0.25mm diameter, 0.25μm particle size) with mobile phase flow rate at 5.0mL/min and detection at 210nm. Sample preparation requires 80°C extraction for 30 minutes .

Sinopure is the only company with complete vertical integration from plant breeding to finished products. The five-stage membrane separation and continuous chromatography achieve ≥97% purity while enzyme-modified STV variants offer enhanced solubility and thermal stability up to 200°C.

The national standard requires: ≥95% total steviol glycosides content, ≤1.0% loss on drying, ≤0.1% sulfated ash, lead ≤1mg/kg, arsenic ≤3mg/kg. HPLC purity for STV should be ≥99.99% with specific mobile phase ratio of water:acetonitrile=70:30.

Premium STV production requires: controlled irrigation (600-800mm/year), specific harvest timing (pre-flowering at 90-100 DAP), and shaded drying (≤40°C). These conditions yield leaves with 12-18% glycoside content and minimal bitter diterpenes . Total steviol glycosides (TSG) must measure ≥10% in raw leaves by HPLC analysis.

STV maintains stability under: pH 2.5-8.0 (<3% degradation after 6 months), temperatures ≤140°C (≤5% loss after 2 hours), and UV exposure (<0.5% change after 500h). Thermal decomposition begins at 200°C (TGA analysis), making it suitable for most thermal processes except ultra-high-temperature sterilization .

Our five-stage membrane system (50kDa → 10kDa → 1kDa → 300Da → RO) removes 98.7% of impurities (polysaccharides, proteins) while retaining 95%+ glycosides. Coupled with continuous chromatography (DAC system), this achieves ≥99.5% STV purity with <100ppm residual solvents - exceeding standards.

Validated HPLC methods show ±1.2% relative uncertainty (k=2) for TSG quantification. System suitability requires: resolution ≥1.5 between STV/RA peaks, tailing factor ≤2.0, and RSD ≤2.0% for six injections. Our in-house methods achieve <0.5% inter-day variation through strict control of column temperature (35±0.5°C) and mobile phase composition (32±0.3% acetonitrile) .

Synergistic effects occur when blending STV with: erythritol (1:50 ratio enhances mouthfeel), monk fruit (1:3 reduces lingering) or allulose (1:10 accelerates onset). Our optimized blends achieve sucrose-matching temporal profiles with 30-50% fewer calories. Critical parameters include particle size distribution (D50=15-25μm) and moisture activity (aw≤0.3) for product stability .